127 research outputs found
Understanding the importance of quality control and quality assurance in preclinical PET/CT imaging
The fundamental principle of experimental design is to ensure efficiency and efficacy of the performed experiments. Therefore, it behoves the researcher to gain knowledge of the technological equipment to be used. This should include an understanding of the instrument quality control and assurance requirements to avoid inadequate or spurious results due to instrumentation bias whilst improving reproducibility. Here, the important role of preclinical positron emission tomography/computed tomography and the scanner's required quality control and assurance is presented along with the suggested guidelines for quality control and assurance. There are a multitude of factors impeding the continuity and reproducibility of preclinical research data within a single laboratory as well as across laboratories. A more robust experimental design incorporating validation or accreditation of the scanner performance can reduce inconsistencies. Moreover, the well-being and welfare of the laboratory animals being imaged is prime justification for refining experimental designs to include verification of instrumentation quality control and assurance. Suboptimal scanner performance is not consistent with the 3R principle (Replacement, Reduction, and Refinement) and potentially subjects animals to unnecessary harm. Thus, quality assurance and control should be of paramount interest to any scientist conducting animal studies. For this reason, through this work, we intend to raise the awareness of researchers using PET/CT regarding quality control/quality assurance (QC/QA) guidelines and instil the importance of confirming that these are routinely followed. We introduce a basic understanding of the PET/CT scanner, present the purpose of QC/QA as well as provide evidence of imaging data biases caused by lack of QC/QA. This is shown through a review of the literature, QC/QA accepted standard protocols and our research. We also want to encourage researchers to have discussions with the PET/CT facilities manager and/or technicians to develop the optimal designed PET/CT experiment for obtaining their scientific objective. Additionally, this work provides an easy gateway to multiple resources not only for PET/CT knowledge but for guidelines and assistance in preclinical experimental design to enhance scientific integrity of the data and ensure animal welfare
Muon Acceleration in Cosmic-ray Sources
Many models of ultra-high energy cosmic-ray production involve acceleration
in linear accelerators located in Gamma-Ray Bursts magnetars, or other sources.
These source models require very high accelerating gradients, keV/cm,
with the minimum gradient set by the length of the source. At gradients above
1.6 keV/cm, muons produced by hadronic interactions undergo significant
acceleration before they decay. This acceleration hardens the neutrino energy
spectrum and greatly increases the high-energy neutrino flux. We rule out many
models of linear acceleration, setting strong constraints on plasma wakefield
accelerators and on models for sources like Gamma Ray Bursts and magnetars.Comment: 5 pgs. submitted for publicatio
First Study of Combined Blazar Light Curves with FACT and HAWC
For studying variable sources like blazars, it is crucial to achieve unbiased
monitoring, either with dedicated telescopes in pointing mode or survey
instruments. At TeV energies, the High Altitude Water Cherenkov (HAWC)
observatory monitors approximately two thirds of the sky every day. It uses the
water Cherenkov technique, which provides an excellent duty cycle independent
of weather and season. The First G-APD Cherenkov Telescope (FACT) monitors a
small sample of sources with better sensitivity, using the imaging air
Cherenkov technique. Thanks to its camera with silicon-based photosensors, FACT
features an excellent detector performance and stability and extends its
observations to times with strong moonlight, increasing the duty cycle compared
to other imaging air Cherenkov telescopes. As FACT and HAWC have overlapping
energy ranges, a joint study can exploit the longer daily coverage given that
the observatories' locations are offset by 5.3 hours. Furthermore, the better
sensitivity of FACT adds a finer resolution of features on hour-long time
scales, while the continuous duty cycle of HAWC ensures evenly sampled
long-term coverage. Thus, the two instruments complement each other to provide
a more complete picture of blazar variability. In this presentation, the first
joint study of light curves from the two instruments will be shown, correlating
long-term measurements with daily sampling between air and water Cherenkov
telescopes. The presented results focus on the study of the variability of the
bright blazars Mrk 421 and Mrk 501 during the last two years featuring various
flaring activities.Comment: 6 pages, 2 figures. Contribution to the 6th International Symposium
on High Energy Gamma-Ray Astronomy (Gamma2016), Heidelberg, Germany. To be
published in the AIP Conference Proceeding
Optimizing SUV Analysis: A Multicenter Study on Preclinical FDG-PET/CT Highlights the Impact of Standardization
PURPOSE: Preclinical imaging, with translational potential, lacks a standardized method for defining volumes of interest (VOIs), impacting data reproducibility. The aim of this study was to determine the interobserver variability of VOI sizes and standard uptake values (SUV mean and SUV max) of different organs using the same [ 18F]FDG-PET and PET/CT datasets analyzed by multiple observers. In addition, the effect of a standardized analysis approach was evaluated. PROCEDURES: In total, 12 observers (4 beginners and 8 experts) analyzed identical preclinical [ 18F]FDG-PET-only and PET/CT datasets according to their local default image analysis protocols for multiple organs. Furthermore, a standardized protocol was defined, including detailed information on the respective VOI size and position for multiple organs, and all observers reanalyzed the PET/CT datasets following this protocol. RESULTS: Without standardization, significant differences in the SUV mean and SUV max were found among the observers. Coregistering CT images with PET images improved the comparability to a limited extent. The introduction of a standardized protocol that details the VOI size and position for multiple organs reduced interobserver variability and enhanced comparability. CONCLUSIONS: The protocol offered clear guidelines and was particularly beneficial for beginners, resulting in improved comparability of SUV mean and SUV max values for various organs. The study suggested that incorporating an additional VOI template could further enhance the comparability of the findings in preclinical imaging analyses. </p
Correlated Multimodal Imaging in Life Sciences:Expanding the Biomedical Horizon
International audienceThe frontiers of bioimaging are currently being pushed toward the integration and correlation of several modalities to tackle biomedical research questions holistically and across multiple scales. Correlated Multimodal Imaging (CMI) gathers information about exactly the same specimen with two or more complementary modalities that-in combination-create a composite and complementary view of the sample (including insights into structure, function, dynamics and molecular composition). CMI allows to describe biomedical processes within their overall spatio-temporal context and gain a mechanistic understanding of cells, tissues, diseases or organisms by untangling their molecular mechanisms within their native environment. The two best-established CMI implementations for small animals and model organisms are hardware-fused platforms in preclinical imaging (Hybrid Imaging) and Correlated Light and Electron Microscopy (CLEM) in biological imaging. Although the merits of Preclinical Hybrid Imaging (PHI) and CLEM are well-established, both approaches would benefit from standardization of protocols, ontologies and data handling, and the development of optimized and advanced implementations. Specifically, CMI pipelines that aim at bridging preclinical and biological imaging beyond CLEM and PHI are rare but bear great potential to substantially advance both bioimaging and biomedical research. CMI faces three mai
Contribución al conocimiento de Porosagrotis gypaetina (Guen.) (Lep.:Noctuidae)
p.15-22Este trabajo tiene por finalidad brindar una descripcion detallada de los diferentes estados de desarrollo, asi como de los estadios larvales, de Porosagrotis gypaetina (Guen.) y estimar sus principales parametros biologicos. Se trata de una oruga conocida vulgarmente como gusano pardo que frecuenta cultivos de alfalfa, trebol bianco, maiz y girasol y determinadas malezas. Los caracteres considerados para su identificacion fueron, en el huevo: numero y distribucion de costas; en la larva: pigmentacion, distribucion de manchas y cerdas corporales; en la pupa: tamaño, forma y color y caracteristicas del cremaster; y en el adulto: ubicacion y coloracion de maculas y nervaduras alares. La emergencia de imagos alcanzo su maximo en abril y mayo. El periodo embrionario se completo en 22 a 26 dias. Aproximadamente la mitad de las larvas cumplieron su ciclo en 6 estadios y las restantes en 7; la duracion total del periodo larval fue de 134 a 141 dias, sin considerar la forma prepupal e independientemente del numero de estadios. Las orugas permanecieron como prepupas durante la temporada estival (aproximadamente 161 dias). El estado pupal duro 40 a 57 dias. Las observaciones realizadas permiten expresar que, inediante los caracteres descriptos, es factible reconocer la especie a traves no solo de los adultos, sino de sus estados inmaduros. Posee una sola generacion anual; transcurre el inviemo como larva; el daño tipico de corte lo produce a partir del cuarto estadio larval
High-energy neutrinos in the context of multimessenger physics
The field of astroparticle physics is currently developing rapidly, since new
experiments challenge our understanding of the investigated processes. Three
messengers can be used to extract information on the properties of
astrophysical sources: photons, charged Cosmic Rays and neutrinos. This review
focuses on high-energy neutrinos (E>100 GeV) with the main topics as follows.
The production mechanism of high-energy neutrinos in astrophysical shocks.
The connection between the observed photon spectra and charged Cosmic Rays is
described and the source properties as they are known from photon observations
and from charged Cosmic Rays are presented.
High-energy neutrino detection. Current detection methods are described and
the status of the next generation neutrino telescopes are reviewed. In
particular, water and ice Cherenkov detectors as well as radio measurements in
ice and with balloon experiments are presented. In addition, future
perspectives for optical, radio and acoustic detection of neutrinos are
reviewed.
Sources of neutrino emission. The main source classes are reviewed, i.e.
galactic sources, Active Galactic Nuclei, starburst galaxies and Gamma Ray
Bursts. The interaction of high energy protons with the cosmic microwave
background implies the production of neutrinos, referred to as GZK neutrinos.
Implications of neutrino flux limits. Recent limits given by the AMANDA
experiment and their implications regarding the physics of the sources are
presented.Comment: accepted for publication by Physics Reports, 127 pages, 29 figure
Long-term monitoring of bright blazars in the multi-GeV to TeV range with FACT
Blazars like Markarian 421 or Markarian 501 are active galactic nuclei (AGN), with their jets orientated towards the observer. They are among the brightest objects in the very high energy (VHE) gamma ray regime (>100 GeV). Their emitted gamma-ray fluxes are extremely variable, with changing activity levels on timescales between minutes, months, and even years. Several questions are part of the current research, such as the question of the emission regions or the engine of the AGN and the particle acceleration. A dedicated longterm monitoring program is necessary to investigate the properties of blazars in detail. A densely sampled and unbiased light curve allows for observation of both high and low states of the sources, and the combination with multi-wavelength observation could contribute to the answer of several questions mentioned above. FACT (First G-APD Cherenkov Telescope) is the first operational telescope using silicon photomultiplier (SiPM, also known as Geigermode—Avalanche Photo Diode, G-APD) as photon detectors. SiPM have a very homogenous and stable longterm performance, and allow operation even during full moon without any filter, leading to a maximal duty cycle for an Imaging Air Cherenkov Telescope (IACT). Hence, FACT is an ideal device for such a longterm monitoring of bright blazars. A small set of sources (e.g., Markarian 421, Markarian 501, 1ES 1959+650, and 1ES 2344+51.4) is currently being monitored. In this contribution, the FACT telescope and the concept of longterm monitoring of bright blazars will be introduced. The results of the monitoring program will be shown, and the advantages of densely sampled and unbiased light curves will be discussed
- …